tcr-alpha and beta sequences Search Results


mouse t cell receptor beta primers  (iRepertoire Inc)
90
iRepertoire Inc mouse t cell receptor beta primers
Mouse T Cell Receptor Beta Primers, supplied by iRepertoire Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse t cell receptor beta primers/product/iRepertoire Inc
Average 90 stars, based on 1 article reviews
mouse t cell receptor beta primers - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
insert coding for the tcr-α and -β chains  (GenScript corporation)
90
GenScript corporation insert coding for the tcr-α and -β chains
Insert Coding For The Tcr α And β Chains, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/insert coding for the tcr-α and -β chains/product/GenScript corporation
Average 90 stars, based on 1 article reviews
insert coding for the tcr-α and -β chains - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
genomic dna deep sequencing of tcr α and β chains  (Adaptive Biotechnologies Corp)
90
Adaptive Biotechnologies Corp genomic dna deep sequencing of tcr α and β chains
Genomic Dna Deep Sequencing Of Tcr α And β Chains, supplied by Adaptive Biotechnologies Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genomic dna deep sequencing of tcr α and β chains/product/Adaptive Biotechnologies Corp
Average 90 stars, based on 1 article reviews
genomic dna deep sequencing of tcr α and β chains - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
next generation sequencing of alpha and beta chains of the tcr repertoire  (Illumina Inc)
90
Illumina Inc next generation sequencing of alpha and beta chains of the tcr repertoire
Spearman correlation analyses of transcriptomic and <t> TCR repertoire </t> measurements with demographic and clinical parameters.
Next Generation Sequencing Of Alpha And Beta Chains Of The Tcr Repertoire, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/next generation sequencing of alpha and beta chains of the tcr repertoire/product/Illumina Inc
Average 90 stars, based on 1 article reviews
next generation sequencing of alpha and beta chains of the tcr repertoire - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
genes encoding hla-dq8-c-pep/hip restricted tcr α- and β-chains  (Twist Bioscience)
90
Twist Bioscience genes encoding hla-dq8-c-pep/hip restricted tcr α- and β-chains
Spearman correlation analyses of transcriptomic and <t> TCR repertoire </t> measurements with demographic and clinical parameters.
Genes Encoding Hla Dq8 C Pep/Hip Restricted Tcr α And β Chains, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genes encoding hla-dq8-c-pep/hip restricted tcr α- and β-chains/product/Twist Bioscience
Average 90 stars, based on 1 article reviews
genes encoding hla-dq8-c-pep/hip restricted tcr α- and β-chains - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
multiplexed pcr amplification of mrna or gdna of heavy/light chains (bcr/ig) and alpha/beta chains (tcr)  (iRepertoire Inc)
90
iRepertoire Inc multiplexed pcr amplification of mrna or gdna of heavy/light chains (bcr/ig) and alpha/beta chains (tcr)
Representative methods to obtain immune receptor repertoire data
Multiplexed Pcr Amplification Of Mrna Or Gdna Of Heavy/Light Chains (Bcr/Ig) And Alpha/Beta Chains (Tcr), supplied by iRepertoire Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multiplexed pcr amplification of mrna or gdna of heavy/light chains (bcr/ig) and alpha/beta chains (tcr)/product/iRepertoire Inc
Average 90 stars, based on 1 article reviews
multiplexed pcr amplification of mrna or gdna of heavy/light chains (bcr/ig) and alpha/beta chains (tcr) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
expression constructs for soluble tcr alpha and beta chains  (GenScript corporation)
90
GenScript corporation expression constructs for soluble tcr alpha and beta chains
Representative methods to obtain immune receptor repertoire data
Expression Constructs For Soluble Tcr Alpha And Beta Chains, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression constructs for soluble tcr alpha and beta chains/product/GenScript corporation
Average 90 stars, based on 1 article reviews
expression constructs for soluble tcr alpha and beta chains - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
neoantigen-specific tcr beta and alpha chains  (Adaptive Biotechnologies Corp)
90
Adaptive Biotechnologies Corp neoantigen-specific tcr beta and alpha chains
Transfer <t>of</t> <t>U2AF1</t> Q157R neoantigen-specific TCR confers specificity and function. (A) Representative flow plots demonstrating expression of D1.C32 U2AF1 Q157R /A*33:03-specific TCR (TCR32) transduced (TD) into primary human CD8 + T cells after CRISPR/Cas9-mediated knock-out of endogenous TCR <t>alpha</t> and beta chains (TCRko). Staining for the RQR8 transduction marker (CD34), left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. (B) Representative flow plots demonstrating expression of clone D2.C177 U2AF1 Q157R /A*33:01-specific TCR (TCR177) TD into TCRko primary human CD8 + T cells. CD34 staining, left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. For A and B top, untransduced (UTD); middle, TCR TD; bottom, parental clone. (C) Testing of TCR32 TD T cells (dark red, solid) with parental clone control (light red, dashed) and (D) testing of TCR177 TD T cells (dark purple, solid) and parental clone control (light purple, dashed), for specific lytic activity in peptide titration CRA. For both C and D technical triplicates shown. (E) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR32 TD T cells (dark red) or parental clone control (light red) in flow cytometry-based cytotoxicity assay. (F) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR177 TD T cells (dark purple) or parental clone control (light purple) in flow cytometry-based cytotoxicity assay. For both E and F >3 technical replicates; top plot, U2AF1 Q157R minigene TD TF-1; bottom plots, wild-type (WT) U2AF1 minigene TD TF-1. (G) Per cent survival of U2AF1 Q157R / HLA-A*33:01 + primary AML (left) or wild-type U2AF1 (Q157R-)/HLA-A*33:03 + primary AML (right) co-cultured with either TCR32 TD T cells (red) or TCR177 TD T cells (purple) in flow cytometry-based cytotoxicity assay. Data from >3 technical replicates. Mean and SEM are shown. AML, acute myeloid leukemia; HLA, human leukocyte antigen; pHLA, peptide-HLA; TCR, T-cell receptor.
Neoantigen Specific Tcr Beta And Alpha Chains, supplied by Adaptive Biotechnologies Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/neoantigen-specific tcr beta and alpha chains/product/Adaptive Biotechnologies Corp
Average 90 stars, based on 1 article reviews
neoantigen-specific tcr beta and alpha chains - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
tcr-α (tra) and tcr-β (trb) deep sequencing  (Adaptive Biotechnologies Corp)
90
Adaptive Biotechnologies Corp tcr-α (tra) and tcr-β (trb) deep sequencing
Transfer <t>of</t> <t>U2AF1</t> Q157R neoantigen-specific TCR confers specificity and function. (A) Representative flow plots demonstrating expression of D1.C32 U2AF1 Q157R /A*33:03-specific TCR (TCR32) transduced (TD) into primary human CD8 + T cells after CRISPR/Cas9-mediated knock-out of endogenous TCR <t>alpha</t> and beta chains (TCRko). Staining for the RQR8 transduction marker (CD34), left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. (B) Representative flow plots demonstrating expression of clone D2.C177 U2AF1 Q157R /A*33:01-specific TCR (TCR177) TD into TCRko primary human CD8 + T cells. CD34 staining, left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. For A and B top, untransduced (UTD); middle, TCR TD; bottom, parental clone. (C) Testing of TCR32 TD T cells (dark red, solid) with parental clone control (light red, dashed) and (D) testing of TCR177 TD T cells (dark purple, solid) and parental clone control (light purple, dashed), for specific lytic activity in peptide titration CRA. For both C and D technical triplicates shown. (E) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR32 TD T cells (dark red) or parental clone control (light red) in flow cytometry-based cytotoxicity assay. (F) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR177 TD T cells (dark purple) or parental clone control (light purple) in flow cytometry-based cytotoxicity assay. For both E and F >3 technical replicates; top plot, U2AF1 Q157R minigene TD TF-1; bottom plots, wild-type (WT) U2AF1 minigene TD TF-1. (G) Per cent survival of U2AF1 Q157R / HLA-A*33:01 + primary AML (left) or wild-type U2AF1 (Q157R-)/HLA-A*33:03 + primary AML (right) co-cultured with either TCR32 TD T cells (red) or TCR177 TD T cells (purple) in flow cytometry-based cytotoxicity assay. Data from >3 technical replicates. Mean and SEM are shown. AML, acute myeloid leukemia; HLA, human leukocyte antigen; pHLA, peptide-HLA; TCR, T-cell receptor.
Tcr α (Tra) And Tcr β (Trb) Deep Sequencing, supplied by Adaptive Biotechnologies Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tcr-α (tra) and tcr-β (trb) deep sequencing/product/Adaptive Biotechnologies Corp
Average 90 stars, based on 1 article reviews
tcr-α (tra) and tcr-β (trb) deep sequencing - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
tcr-α–, tcr-β–, and tcr-α/β–transgenic nod mice  (Jackson Laboratory)
90
Jackson Laboratory tcr-α–, tcr-β–, and tcr-α/β–transgenic nod mice
Transfer <t>of</t> <t>U2AF1</t> Q157R neoantigen-specific TCR confers specificity and function. (A) Representative flow plots demonstrating expression of D1.C32 U2AF1 Q157R /A*33:03-specific TCR (TCR32) transduced (TD) into primary human CD8 + T cells after CRISPR/Cas9-mediated knock-out of endogenous TCR <t>alpha</t> and beta chains (TCRko). Staining for the RQR8 transduction marker (CD34), left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. (B) Representative flow plots demonstrating expression of clone D2.C177 U2AF1 Q157R /A*33:01-specific TCR (TCR177) TD into TCRko primary human CD8 + T cells. CD34 staining, left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. For A and B top, untransduced (UTD); middle, TCR TD; bottom, parental clone. (C) Testing of TCR32 TD T cells (dark red, solid) with parental clone control (light red, dashed) and (D) testing of TCR177 TD T cells (dark purple, solid) and parental clone control (light purple, dashed), for specific lytic activity in peptide titration CRA. For both C and D technical triplicates shown. (E) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR32 TD T cells (dark red) or parental clone control (light red) in flow cytometry-based cytotoxicity assay. (F) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR177 TD T cells (dark purple) or parental clone control (light purple) in flow cytometry-based cytotoxicity assay. For both E and F >3 technical replicates; top plot, U2AF1 Q157R minigene TD TF-1; bottom plots, wild-type (WT) U2AF1 minigene TD TF-1. (G) Per cent survival of U2AF1 Q157R / HLA-A*33:01 + primary AML (left) or wild-type U2AF1 (Q157R-)/HLA-A*33:03 + primary AML (right) co-cultured with either TCR32 TD T cells (red) or TCR177 TD T cells (purple) in flow cytometry-based cytotoxicity assay. Data from >3 technical replicates. Mean and SEM are shown. AML, acute myeloid leukemia; HLA, human leukocyte antigen; pHLA, peptide-HLA; TCR, T-cell receptor.
Tcr α–, Tcr β–, And Tcr α/β–Transgenic Nod Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tcr-α–, tcr-β–, and tcr-α/β–transgenic nod mice/product/Jackson Laboratory
Average 90 stars, based on 1 article reviews
tcr-α–, tcr-β–, and tcr-α/β–transgenic nod mice - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
synthetic alpha and beta vdj fragments of the variable region of the tcr  (Twist Bioscience)
90
Twist Bioscience synthetic alpha and beta vdj fragments of the variable region of the tcr
Transfer <t>of</t> <t>U2AF1</t> Q157R neoantigen-specific TCR confers specificity and function. (A) Representative flow plots demonstrating expression of D1.C32 U2AF1 Q157R /A*33:03-specific TCR (TCR32) transduced (TD) into primary human CD8 + T cells after CRISPR/Cas9-mediated knock-out of endogenous TCR <t>alpha</t> and beta chains (TCRko). Staining for the RQR8 transduction marker (CD34), left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. (B) Representative flow plots demonstrating expression of clone D2.C177 U2AF1 Q157R /A*33:01-specific TCR (TCR177) TD into TCRko primary human CD8 + T cells. CD34 staining, left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. For A and B top, untransduced (UTD); middle, TCR TD; bottom, parental clone. (C) Testing of TCR32 TD T cells (dark red, solid) with parental clone control (light red, dashed) and (D) testing of TCR177 TD T cells (dark purple, solid) and parental clone control (light purple, dashed), for specific lytic activity in peptide titration CRA. For both C and D technical triplicates shown. (E) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR32 TD T cells (dark red) or parental clone control (light red) in flow cytometry-based cytotoxicity assay. (F) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR177 TD T cells (dark purple) or parental clone control (light purple) in flow cytometry-based cytotoxicity assay. For both E and F >3 technical replicates; top plot, U2AF1 Q157R minigene TD TF-1; bottom plots, wild-type (WT) U2AF1 minigene TD TF-1. (G) Per cent survival of U2AF1 Q157R / HLA-A*33:01 + primary AML (left) or wild-type U2AF1 (Q157R-)/HLA-A*33:03 + primary AML (right) co-cultured with either TCR32 TD T cells (red) or TCR177 TD T cells (purple) in flow cytometry-based cytotoxicity assay. Data from >3 technical replicates. Mean and SEM are shown. AML, acute myeloid leukemia; HLA, human leukocyte antigen; pHLA, peptide-HLA; TCR, T-cell receptor.
Synthetic Alpha And Beta Vdj Fragments Of The Variable Region Of The Tcr, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synthetic alpha and beta vdj fragments of the variable region of the tcr/product/Twist Bioscience
Average 90 stars, based on 1 article reviews
synthetic alpha and beta vdj fragments of the variable region of the tcr - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
codon-optimized tcr-α and -β fragments  (GenScript corporation)
90
GenScript corporation codon-optimized tcr-α and -β fragments
Transfer <t>of</t> <t>U2AF1</t> Q157R neoantigen-specific TCR confers specificity and function. (A) Representative flow plots demonstrating expression of D1.C32 U2AF1 Q157R /A*33:03-specific TCR (TCR32) transduced (TD) into primary human CD8 + T cells after CRISPR/Cas9-mediated knock-out of endogenous TCR <t>alpha</t> and beta chains (TCRko). Staining for the RQR8 transduction marker (CD34), left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. (B) Representative flow plots demonstrating expression of clone D2.C177 U2AF1 Q157R /A*33:01-specific TCR (TCR177) TD into TCRko primary human CD8 + T cells. CD34 staining, left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. For A and B top, untransduced (UTD); middle, TCR TD; bottom, parental clone. (C) Testing of TCR32 TD T cells (dark red, solid) with parental clone control (light red, dashed) and (D) testing of TCR177 TD T cells (dark purple, solid) and parental clone control (light purple, dashed), for specific lytic activity in peptide titration CRA. For both C and D technical triplicates shown. (E) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR32 TD T cells (dark red) or parental clone control (light red) in flow cytometry-based cytotoxicity assay. (F) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR177 TD T cells (dark purple) or parental clone control (light purple) in flow cytometry-based cytotoxicity assay. For both E and F >3 technical replicates; top plot, U2AF1 Q157R minigene TD TF-1; bottom plots, wild-type (WT) U2AF1 minigene TD TF-1. (G) Per cent survival of U2AF1 Q157R / HLA-A*33:01 + primary AML (left) or wild-type U2AF1 (Q157R-)/HLA-A*33:03 + primary AML (right) co-cultured with either TCR32 TD T cells (red) or TCR177 TD T cells (purple) in flow cytometry-based cytotoxicity assay. Data from >3 technical replicates. Mean and SEM are shown. AML, acute myeloid leukemia; HLA, human leukocyte antigen; pHLA, peptide-HLA; TCR, T-cell receptor.
Codon Optimized Tcr α And β Fragments, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/codon-optimized tcr-α and -β fragments/product/GenScript corporation
Average 90 stars, based on 1 article reviews
codon-optimized tcr-α and -β fragments - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Spearman correlation analyses of transcriptomic and TCR repertoire measurements with demographic and clinical parameters.

Journal: Frontiers in Immunology

Article Title: Persistent T Cell Repertoire Perturbation and T Cell Activation in HIV After Long Term Treatment

doi: 10.3389/fimmu.2021.634489

Figure Lengend Snippet: Spearman correlation analyses of transcriptomic and TCR repertoire measurements with demographic and clinical parameters.

Article Snippet: Total RNA was extracted from blood and subjected to next generation sequencing of alpha and beta chains of the TCR repertoire on the Illumina NextSeq platform, using an established quantitative TCR sequencing pipeline that integrates experimental library preparation and computational analysis with Decombinator (version 3.1) ( , ).

Techniques: Sampling, Modification

Representative methods to obtain immune receptor repertoire data

Journal: Pathology International

Article Title: Immune repertoire profiling for disease pathobiology

doi: 10.1111/pin.13284

Figure Lengend Snippet: Representative methods to obtain immune receptor repertoire data

Article Snippet: iRepertoire , Multiplexed PCR amplification of mRNA or gDNA of heavy/light chains (BCR/Ig) and alpha/beta chains (TCR) , Deeper acquisition of data (10 5 ~10 6 repertoires) , Combinations of heavy/light (BCR/Ig) and alpha/beta (TCR) chains are uninformative, except for dominant clones. , https://irepertoire.com/.

Techniques: Amplification, Sequencing, Clone Assay

Transfer of U2AF1 Q157R neoantigen-specific TCR confers specificity and function. (A) Representative flow plots demonstrating expression of D1.C32 U2AF1 Q157R /A*33:03-specific TCR (TCR32) transduced (TD) into primary human CD8 + T cells after CRISPR/Cas9-mediated knock-out of endogenous TCR alpha and beta chains (TCRko). Staining for the RQR8 transduction marker (CD34), left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. (B) Representative flow plots demonstrating expression of clone D2.C177 U2AF1 Q157R /A*33:01-specific TCR (TCR177) TD into TCRko primary human CD8 + T cells. CD34 staining, left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. For A and B top, untransduced (UTD); middle, TCR TD; bottom, parental clone. (C) Testing of TCR32 TD T cells (dark red, solid) with parental clone control (light red, dashed) and (D) testing of TCR177 TD T cells (dark purple, solid) and parental clone control (light purple, dashed), for specific lytic activity in peptide titration CRA. For both C and D technical triplicates shown. (E) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR32 TD T cells (dark red) or parental clone control (light red) in flow cytometry-based cytotoxicity assay. (F) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR177 TD T cells (dark purple) or parental clone control (light purple) in flow cytometry-based cytotoxicity assay. For both E and F >3 technical replicates; top plot, U2AF1 Q157R minigene TD TF-1; bottom plots, wild-type (WT) U2AF1 minigene TD TF-1. (G) Per cent survival of U2AF1 Q157R / HLA-A*33:01 + primary AML (left) or wild-type U2AF1 (Q157R-)/HLA-A*33:03 + primary AML (right) co-cultured with either TCR32 TD T cells (red) or TCR177 TD T cells (purple) in flow cytometry-based cytotoxicity assay. Data from >3 technical replicates. Mean and SEM are shown. AML, acute myeloid leukemia; HLA, human leukocyte antigen; pHLA, peptide-HLA; TCR, T-cell receptor.

Journal: Journal for Immunotherapy of Cancer

Article Title: Discovery of U2AF1 neoantigens in myeloid neoplasms

doi: 10.1136/jitc-2023-007490

Figure Lengend Snippet: Transfer of U2AF1 Q157R neoantigen-specific TCR confers specificity and function. (A) Representative flow plots demonstrating expression of D1.C32 U2AF1 Q157R /A*33:03-specific TCR (TCR32) transduced (TD) into primary human CD8 + T cells after CRISPR/Cas9-mediated knock-out of endogenous TCR alpha and beta chains (TCRko). Staining for the RQR8 transduction marker (CD34), left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. (B) Representative flow plots demonstrating expression of clone D2.C177 U2AF1 Q157R /A*33:01-specific TCR (TCR177) TD into TCRko primary human CD8 + T cells. CD34 staining, left, and U2AF1 Q157R /A*33:03-pHLA tetramer, right. For A and B top, untransduced (UTD); middle, TCR TD; bottom, parental clone. (C) Testing of TCR32 TD T cells (dark red, solid) with parental clone control (light red, dashed) and (D) testing of TCR177 TD T cells (dark purple, solid) and parental clone control (light purple, dashed), for specific lytic activity in peptide titration CRA. For both C and D technical triplicates shown. (E) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR32 TD T cells (dark red) or parental clone control (light red) in flow cytometry-based cytotoxicity assay. (F) Per cent survival of TF-1 minigene-transduced cells co-cultured with TCR177 TD T cells (dark purple) or parental clone control (light purple) in flow cytometry-based cytotoxicity assay. For both E and F >3 technical replicates; top plot, U2AF1 Q157R minigene TD TF-1; bottom plots, wild-type (WT) U2AF1 minigene TD TF-1. (G) Per cent survival of U2AF1 Q157R / HLA-A*33:01 + primary AML (left) or wild-type U2AF1 (Q157R-)/HLA-A*33:03 + primary AML (right) co-cultured with either TCR32 TD T cells (red) or TCR177 TD T cells (purple) in flow cytometry-based cytotoxicity assay. Data from >3 technical replicates. Mean and SEM are shown. AML, acute myeloid leukemia; HLA, human leukocyte antigen; pHLA, peptide-HLA; TCR, T-cell receptor.

Article Snippet: U2AF1 Q157R neoantigen-specific TCR beta and alpha chains were sequenced by next-generation sequencing (Adaptive Biotechnologies, Takara Bio USA) as detailed in .

Techniques: Expressing, CRISPR, Knock-Out, Staining, Transduction, Marker, Control, Activity Assay, Titration, Cell Culture, Flow Cytometry, Cytotoxicity Assay